Microarray Analysis of the zms1 Knockout Mutant's Gene Expression Levels in Sacchromyces cerevisiae

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Abstract:

    Sacchromyces cerevisiae is considered an ideal model system for eukaryotic studies to study oxidative stress reactions to free radicals. Microarray techniques were used to determine the  ∆ZMS1 gene expression profile responsible for the suppressing the mutant phenotypes of a zwf1 gene in S. cerevisiae.  MAGIC Tool software was used to analyze data obtained from the microarray . Microarray technology is a rapidly advancing field which gives insights into gene expression profiles, providing guidelines for future work.  When looking at genes in grids 3 and 4 for the ΔZMS1 mutant compared with the wild type gene expression, we found five genes which were up regulated and five genes which were down regulated.  Of the ten selected genes from ∆ZMS1 that had consistent expression level changes (at least 5 of the 7 slides), six of the genes have no known molecular function and five of these genes have not been determined as to which cellular components they comprise. Gene YPL051W, an intracellular protein transporter with monomeric GTase activity, was found to be up-regulated.  Gene YDR165W, responsible tRNA methylation and YAL017W, a gene responsible for protein amino acid phosphorylation, were found to be down-regulated.  Most significantly, gene YML120C, a protein involved with the oxidative phosphorylation of NADH to ubiquinone in complex I of the electron transport chain was found to be down-regulated. 

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Noble Egekwu - egekwuni@jmu.edu    Karl Gorzelnik - gorzelkv@jmu.edu    Jonathan Baugher - baughejl@jmu.edu