Investigation of Oxidative Stress
in ZMS1 and ZMS2 in Saccharomyces
cerevisiaeUsing Microarray Analysis
Introduction Materials and Methods Results Discussion Literature Cited
Oxidative stress plays a devastating role in many pathological conditions and processes due to the absence of reducing agents. Previous studies have suggested that ZMS1 and ZMS2 suppressors in Saccharomyces cerevisiae, or baker's yeast, play a significant role in yeast gene expression of natural antioxidants. Bakers yeast is a model organism to observe knockouts of the genes. Gene regulation by these suppressors may potentially protect the cell from the effects of oxidative stress. Microarray analysis was used to identify gene regulation in an auxotrophic Δzms1/Δzms2 knockout strain. It was hypothesized that genes expressed in the Δzms1/Δzms2 would be up-regulated based on the absence of ΔZMS1 and ΔZMS2. Over expression of these genes results in suppression of the zwf1Δ phenotype. The expression levels of fourteen genes were identified. Overall, ten genes were up regulated and four were down regulated by approximately 13 fold in at least three of the four investigated slides.
Authors :
Brad Heidenthal: Comparison of gene expression levels of ΔZMS1/ΔZMS2 and ΔZMS2 in Saccharomyces cerevisiae
Hilary Jacobson: Making Statistical Analysis Easy with SPSS using ΔZMS1/ ΔZMS2 of s. cerevisiae
Theresa Russo: Gene Regulation in ΔZMS1, ΔZMS2, and ΔZMS1/ ΔZMS2 in Saccharomyces cerevisiae
James Madison University
Last Updated:
12/09/2008