RNA from both mutant ”hexose 20 and Wild Type S. cerevisiae were isolated.  Table 1 shows the OD 600 reading, the purity and the concentrations of both samples from Daniel 2's group.  The RNA from both the mutant and the wild type was run on an agarose gel, figure 1 shows the results of this.  There is a band at 28S, 18S, and 5S for the wild type strain only.  There is also a slight smear in the band at 28S.  Table 2 is a compilation of genes of interest that are involved in the metabolic pathway of sugar transport and metabolism, and their expression in both our group and Daniel 2's group in log₂ form.  Negative numbers imply repression in a gene while positive numbers imply induction.  Figure 2 is a picture of the first grid on slide number 3304.  There are two copies of each gene per grid.  Figure 3 is a comparison of genes that are over expressed 25 fold, however this data was not analyzed in log₂ form, therefore its expression appears to be larger than in actuality.  Figure 4 displays a portion of the clustering analysis performed on the first 1000 genes.   The most similarly expressed genes are shown in this figure.  Table 3 displays the general name and biological function of the four clustered genes determined by expression similarities.

Yeast Strain	Concentration	A260/A280	OD
”hexose 20	0.6 µg/µL	1.56	6.6
Wild Type	0.62 µg/µL	1.56	6.9

Table 1.  The purity, the concentration, and the OD 600 readings of the RNA sample from Daniel 2.  The purity of the sample is determined by the ratio of A260/A280.

Figure 1.  A picture of the gel electrophoresis performed on the rRNA of both the delta hexose20 mutant yeast strain (EBY) and the wild type yeast strain (Cen).  The only lane to show results was the wild type.  There is a slight smear at the 28S band.

Name	Gene numbers	Daniel 1	Daniel 2
STP 1	YDR463W	-2.57999	-1.25694
STP 2	YHR006W	-2.21655	-1.15866
STP 3	YLR375W	-0.41468	0.373519
STP 4	YDL048C	-4.36326	-1.70102
HXT17	YNR072W	-4.97564	-5.33283
GAL2	YLR081W	-3.40368	-2.63651
SNF 3	YDL194W	-0.54436	-0.9027
RGT2	YDL138W	-4.07196	-4.24787
GRR1	YJR090C	-2.1164	-1.87125
HOM 6	YJR139C	1.185951	1.631204

Table 2.  Genes of interest relating to the metabolic processes of the yeast. The numbers represent the ratio data in log₂ transformation of gene expression for the ten genes of both Daniel 1 and Daniel 2 data. Genes STP 1-4 are sugar transport proteins and are in the major superfamily of the hexose transporters.  SNF3 and RGT2 are two glucose transporter-like proteins that serve as glucose sensors for low and high concentrations of glucose, respectively.  GAL2 is a galactose transporter and is able to complement the glucose growth defect of a mutant devoid of hexose transporters.  Grr1 is responsible for glucose induced gene expression.  HXT17 is a hexose transporter and should be knocked out with the mutant.  HOM6 is a homoserine dehydrogenase; it is involved in the electron transport chain which is a part of the metabolic process for the breakdown of sugar into ATP. 

Figure 2.  A picture of a portion of the microarray data from slide number 3304.  This slide used is courtesy of the Biology 480 class of Fall 2004.  The mutant is labeled with red dye and the wild type with green dye.

Figure 3.  This is a comparison of expression levels of both our group and Daniel 2. The top 10 genes with expression above a 25 fold induction were compared.  On the left side of the graph were our group's expression levels for the highly expressed genes.  The right side of the graph contained the expression levels for the data from Daniel 2.

Figure 4.  The portion of four genes from the hierarchical clustering of the first 1000 genes from the merged Daniel 1 and Daniel 2 file, that are most similar to one another.  The genes that are most similar are YLR320W, YAL035W, YHR171W, YGR059W.

Gene #	Standard Name	Biological Function
YLR320W	MMS22	The repair of double-strand breaks in DNA via homologous and non-homologous mechanisms to reform a continuous DNA helix.
YAL035W	FUN12	Protein synthesis initiation; protein-synthesizing GTPase activity
YHR171W	ATG7	The process by which cells digest parts of their own cytoplasm; allows for both recycling of macromolecular constituents under conditions of nutrient limitation and remodeling the intracellular structure for cell differentiation.
YGR059W	SPR3	The assembly and arrangement of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis

Table 3.  The biological functions and standard names of the top four most similar genes as found in Figure 4.