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Image 1.  Gel Electrophoresis of the RNA from yeast cells.  Lane 1 is the wild type yeast RNA and Lane 2 is the mutant RNA.

 

**Microarray for our slide did not work.  We were given Slide #106 from The fall semester of 2004 to analyze.**

 

 

Image 2.  Slide 106 Microarray picture.  The dots represent the areas that the genes bound to the slide.  Red dots represent mutant expression of the gene

and green represent wild-type expression of the gene.

 

Table 1. Common Names and the Description of Selected Genes Used in Comparison of the Data

Common Name Gene Description
ZMS1 YJR127C Zinc finger protein involved in transcriptional control of respiration
ZWF1 YNL241C Glucose-6-phosphate dehydrogenase (G6PD) catalyses the first step of the pentose phosphate pathway involved in oxidative stress.
CUP2 YGL166W Transcription factor involved in oxidative stress
MAC1 YMR021C Transcription factor involved in oxidative stress
YAP1 YML007W Basic leucine zipper transcription factor involved with oxidative tolerance
CAD1 YDR423C Basic leucine zipper transcription involved in multiple stress responses including oxidative stress
HAP1 YLR256W Zinc finger transcription factor involved in the complex regulation of gene expression in response to levels of heme and oxygen
HAP2 YGL237C A transcriptional activator and global regulator of respiratory gene expression
HAP3 YBL021C Subunit of the heme-activated containing sequences contributing to both complex assembly and DNA binding
SOD1 YJR104C Cu, Zn superoxide dismutase
SOD2 YHR008C Manganese-containing superoxide dismutase and protects cells against oxygen toxicity
ATF2 YGR177C Alcohol acetyltransferase, may play a role in steroid detoxification

Table 2.  Ratios of mutant to wild type (red:green) for slide 106 of Fall 2004 microarray.

 

Image 3: The above image is purely for fun. We decided to see how genes with prefix YGL1 correlated.

Image 4: The above image is similar to that of Image 3. Except we chose to observe all those similarities between genes of  prefix YGL2.

Image 5: The above genes are those from Table 1, which we picked because of their relation and role in oxidative stress. As observed, there really isn't that complicated of a relationship.