|
Samples |
Absorbance (750 nm) |
|
Blank |
0.141 |
|
0.125 |
0.251 |
|
0.25 |
0.288 |
|
0.5 |
0.364 |
|
0.75 |
0.426 |
|
1.0 |
0.487 |
|
R30 |
0.280 |
|
R10 |
0.185 |
|
R5 |
0.162 |
|
S30 |
0.225 |
|
S10 |
0.160 |
|
S5 |
0.145 |
|
L30 |
0.814 |
|
L10 |
0.456 |
|
L5 |
0.251 |
Table 1. Absorbencies of Samples and Standards. This table shows the absorbencies from the spectrometer
that were found for the samples and the known standards. The samples being tested are root (R), stem (S), and leaf (L).
For each plant sample (R, S, L) three dilutions were made and include: 1) 30ul of plant protein and 70 ul of QB, 2) 10ul
of plant protein and 90ul of QB and 3) 95 ul of QB and 5 ul of plant protein.
|
Sample |
Concentration (μg/μl) |
Amount loaded on gel (μl) 20/x |
|
R 30 |
0.724 |
27.6 |
|
S 30 |
0.046 |
30 |
|
L 10 |
5.442 |
3.675 |
Table 2. Concentrations and Amounts of Samples Needed. This table shows the calculated concentrations
of the samples. The samples are root (R), stem (S), and leaf (L). Calculations were made using absorbencies from
Table 1 to determine the concentrations for each sample. The equation used for calculations can be found in Figure 1.
The above three samples yielded an absorbance within those set up by the standards; therefore, they were used on
the acrylamide gel. The amount loaded onto the gel in µl was calculated so that 30 µg of protein was loaded per sample onto the gel.