Abstract        Introduction       Methods     Results        Discussion        References

In this experiment, Saccharomyces cerevisiae, commonly known as Baker’s yeast,  was used to compare expression levels between different genes such as ZMS1Δ, ZMS2Δ, ZMS1Δ ZMS2Δ, ZMS1++ (over-expressed ZMS1), and ZMS2++. cDNA was created from RNA isolated from yeast strains zms1Δ, zms2Δ, zms1Δ zms2Δ, ZMS1++, and ZMS2++ and WT. Microarrays were used to analyze gene expression levels. The original microarray did not produce interpretable results so microarray data from a previous year was used. A gel was run to see if RNA degradation was to blame for poor microarray results, however, little to no degradation was observed. Using MAGIC Tool, four genes were identified as being induced and 10 genes identified as repressed in ∆ZMS1 knockout when compared to wild-type expression. Of those genes, five were possibly associated with the oxidative stress pathway and ZMS1 gene. Results from the data analysis of genes responsible for methionine synthesis pathway revealed three genes that were repressed in ZMS1Δ and two genes that were repressed in ZMS2Δ. Also, there was one gene that was induced in ZMS1, ZMS2 double mutant. Out of those genes, two genes are known to be affected by oxidative stress during methionine synthesis.