Microarray Analysis Involving the Comparison of Wild Type and Mutant (ZMS1∆) Strains of  Saccharomyces cerevisiae

Intro      Methods       Results       Discussion       References

Abstract:

    This experiment focused on the analysis of microarray data in the comparison of ZMS1Δ mutant strand of yeast to the wild-type.  This gene is important in that it encodes a transcription factor involved in the oxidative stress pathway. Because most transcriptional factors are involved in multiple synthetic pathways, and can be associated with the cascade of cellular functions a microarray analysis can yield valuable results on how a gene mutation can affect the organism as a whole. From the data collected on the green ZMS1Δ microarray, it was noted that photo bleaching and other systematic errors inherent in microarray analysis was too overwhelming to draw solid conclusions. Nonetheless, a change in expression for the mutant was seen, in particular, one of the the genes involved in oxidative stress was observed to indicate up regulation, but more experimentation and better microarray analysis needs to be completed before jumping to false findings.

Contact Information:

Josh Krueger       Kris Tillett       Gordo McGuire