A Microarray Analysis of ΔZMS1/ΔZMS2

  Abstract 

Microarray analysis was performed on a mutant strain of S. cerevisiae  (ZMS1Δ/ZMS2Δ) to take a look at           complete cellular expression changes.  ZMS1 and ZMS2 genes are both involved in oxidative stress in the yeast    cell.  The mutant knockout strain was directly compared to a microarray of a wild type (WT) S. cerevisiae          (yeast) strain.  cDNA created from isolated and purified RNA from both    strains of yeast were used to hybridize onto the microarray slides.  Cy3 (green) dye was attached to our mutant cDNA while Cy5 (red)   dye was            attached to the WT cDNA.  The pixels of each color are then directly related to the amount of cDNA attached to  the microarray slide, which then directly relates it to the amount of expression for each gene in the yeast cell.        Unfortunately due to human error the data retrieved was unreliable, resulting in no official genetic information.     

Authors: Seth Thompson, Katie Raich, Allyson Samselski

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