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Study of the Transcriptome of ∆ZMS1(∆RSF2) Using Microarray Analysis
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Abstract
ΔZMS1 is a mutant of S. cerevisiae in which the ZMS1 gene, a zinc-finger transcription factor involved in the oxidative stress response, has been deleted. In this study a comparison of the transcriptomes of ΔZMS1 to the wild type in S. cerevisiae indicated thirteen genes which were expressed differently in the ΔZMS1 mutant. Data obtained from microarray slides was normalized using the average negative control ratios as a baseline. Genes of interest were those with ratios greater than three standard deviations from the baseline. Those genes which were induced included genes that were involved in transcription and translation, and in the response to oxidative stress. The induced genes were: YPL167C, YDL005C, YPL181W, YPL188W, YPL190C, YPL204W, YBR102C, YBR116C, and YBR118W. The genes that were repressed included a peroxisome-associated protein. The repressed genes were: YDL065C, YPL278C, YER089C, and YJR042W. In addition there were two unknown genes, one of which was induced and the other was repressed.
Introduction Methods/Materials Results Discussion References
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