We hypothesized that yeast (Saccharomyces cerevisiae) with a double knockout of Δ ZMS1 and Δ ZMS2 would express genes involved in coping with oxidative stress that would not normally be expressed in the wild type. Microarray analysis was used to help determine the role of these two knocked out genes. RNA was isolated and extracted from wild type and mutant yeast samples and reverse transcribed to cDNA to be hybridized on a microarray slide. Microarray data were analyzed and normalized to determine the expression ratios between mutant and wild type genes. Despite problems with dye deterioration due to photobleaching, these analyses yielded several results that warrant further research.
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