MicroArray Analysis of ZMS2 Mutation in Saccharomyces cerevisiae Cells
By Andrew Eshelman Kevin Fedkenheuer and Jaismeen Nijjar
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ABSTRACT
In many organisms, oxidative stress has been linked to harmful diseases, and a full understanding of pathways which can relieve oxidative stress is important in the discovery of novel drug targets. Antioxidants are one class of molecules that prevent cellular damage due to oxidative stress. Many of these antioxidants require a reductive environment to neutralize reactive oxygen species. Mutations in the pentose phosphate pathway can lead to increased oxidative stress. Two genes, ZMS1 and ZMS2, help to relieve cellular oxidative stress caused by mutations in the pentose phosphate pathway. Overexpression of these genes would possibly effect the oxidative stress a cell experiences. Their mechanism was investigated in Saccharomyces cerevisiae utilizing a DNA microarray to compare gene expression in ZMS1 and ZMS2 single knockouts to wildtype yeast. An analysis of the microarray data showed changes in gene expression,both upregulated and downregulated, in many genes related to glycogenesis. These genes can be further examined to gain information regarding the mechanism by which ZMS1 and ZMS2 relieve oxidative stress and to find other genes involved in this pathway.
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