An Experimental Comparison of Gene Expression in ZMS1 and ZMS2 pathways in Wild type and Mutant Yeast Cells
Diane Abadam, Christine Beaudette and Charlie Boucher
abadamde@jmu.edu, beaudeca@jmu.edu and bouchecg@jmu.edu
Abstract:
This experiment was conducted to see the expression pattern of genes controlled by ZMS1 and ZMS2 in wild type and mutant Saccharomyces cerevisiae (bakers yeast) cells. For this study ZMS2 mutant cells were analyzed. Gene expression was compared using microarray analysis. This information is important for understanding the pathway in cells undergoing oxidative stress, which is associated with diseases such as cancer, cardiovascular disease, and neurological diseases (Slecker). From this study genes were identified as being possible up regulated or down regulated by ZMS2. The findings concluded that of the down regulated genes collected and analyzed a consensus sequence was not found that may code for ZMS2. Further examination of other down regulated genes may produce possible consensus sequences for ZMS2.