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A Microarray Investigation of ZMS1 and ZMS2 Mutants in Saccharomyces cerevisiae |
Logan Murphy & Michelle Chen Johnson
Abstract Introduction Materials and Methods Results Discussion Sources Cited
Abstract
Oxidative stress is a contributing factor to many human diseases, including neurodegenerative diseases, cancer, and cardiovascular disease as well as senescence associated with nuclear and mitochondrial DNA damage. To study oxidative stress Dr. Slekar, a James Madison University geneticist, mutated, in Saccharomyces cerevisiae, the ZMS1 and ZMS2 genes which are multicopy supressors of ZWF1. The ZWF1 gene codes for Glucose-6-Phosphate that plays a major role on the pentos phosphate pathway which makes NADPH. Microarray technology uses cDNA libraries printed on a slide, and gives information on up or down regulated genes. From the microarray tree up-regulated YBR284W, YFL049W, and YOR349W and three down regulated genes YFL034C, YKL091C, and YNL145W were found. Two of the up regulated genes have unknown function, though on is involved somehow in moving histones in gene regulation. there were more down-regulated genes than up, but the three most down regulated genes do not seem likely candidates for future research. To have confidence in the microarray analysis more slides with better quality results would be needed and any major results should be confirmed with real time PCR.