METHODS

Plant tissues from hibiscus (Hibiscus rosa-sinensis), mother-in-law's tongue(Sansevieria trifasciata), papyrus (Cyprus alternifolius), and gooseberry(Pereskia aculeate) were obtained from JMU's Biology department's Green House. The species were isolated by cutting the plants into small pieces, lysing the cell wall and extracting the protein.  Protein Isolation

The protein samples were then run on an SDS-PAGE gel and further processed in a Western blot in order to determine presence of protein in the samples and the presence of rubisco in the samples.  Western Blot.  Blot Analysis.

Plant tissue was again finely sliced and CTAB reagent was added to break open the cell walls.  DNA was isolated by precipitation.  DNA Extraction

In order to determine how much rubisco gene was present in each tissue, the rubisco genes were amplified and the results obtained from a Real Time PCR analysis. Real Time PCR

An agarose gel of Running Agarose Gel

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