Rubisco

Results

Leaf Thickness Measurements:

Table 1.

Leaf #	Red Maple	Magnolia	Tulip Tree	Sycamore
1	11	22	7	11
2	17	23	9	14.5
3	8	24	8	13
4	8	24	8	10
5	11	22	8	11
6	13	18	7	14.5
7	7	19	9	9
8	11	18	8	10
9	12	19	8	7
10	5	20	8	8.5
Total	103	209	80	108.5
Average	10.3	20.9	8	10.85

The table contains the measurement results of the thickness of leaves. All measurement are in .001 in.

Protein concentrations after grinding the leaves:

Red Maple: 37.99 mg/ml

Magnolia: 13.36 mg/ml

Tulip Tree: 7.55 mg/ml

Sycamore: 6.58 mg/ml

PCR results:

Figure1.

This graph shows the florecence intencity in each cycle of the PCR. The light green line and the pink line are Magnolia products that were amplified with primer C and primer A. The light blue is the blank for primer A.

Figure 2.

This graph is the melting curve.

Figure 3.

In lanes 1, 2, and 5 Magnolia was run on the gel. In lanes 3, 4, and 6 the blanks were run. Lane 7 was left blank and lanes 8 is the molecular ladder. The magnolia DNA was the only one that was amplified in the PCR reaction.

Protein gel:

Figure 4.

From each plant were loaded in to a polyacrilamide gel. In lane 1, sycamore was loaded. In lane 2, Tulip tree protein was loaded. In lane 3, the Red Maple protein was loaded. In lane 4, the Magnolia was loaded. In lane 5, the molecular weight was loaded.

Western blot membrane:

Figure 5:

Florescent antibodies were used to label the rubisco protein. In lane two Sycamore protein was loaded. In lane three, Tulip tree protein was loaded. In lane four, Red Maple was loaded. In lane five, the Magnolia protein was loaded.

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